- 作者: Wenchang Zhang, Yao Yao, Jingxian Chen, Mingli Wang
- 作者服務機構: Department of Microbiology, Anhui Medical University, 81st Meishan Road, Hefei , Anhui, China
- 中文摘要: --
- 英文摘要:
Background:
The HCMV UL76 gene is a member of UL24 family in herpes virus and encodes a highly conserved herpes virus protein. Inherited from common ancestor, members of Herpes _ UL24 family encode proteins with a conserved N terminal and varied in C terminal region. To define which region (conserved N terminal or unconserved C terminal) of UL76 was responsible for its ability to induce DNA damage and aggresome formation, the wild-type UL76 gene and two deletion mutants were transfected to cells and analyzed by immunofluorescent staining, Western blotting and comet assay.
Results:
We report that the EGFP-fusion proteins present as globular aggresomes and colocalize with γ-H2AX in cells transfected with either pEGFP-UL76 or pEGFP-UL76C. The relative expression level of γ-H2AX and percentage of cells with comet tails were elevated in pEGFP-UL76 or pEGFP-UL76C transfection groups compared to the control.
Conclusion:
Our findings suggest that the unconserved C terminal (not the conserved N terminal) of pUL76 was sufficient to induce DNA damage and aggresome formation in transfected cells. - 中文關鍵字: --
- 英文關鍵字: Human cytomegalovirus (HCMV); Tegument protein; UL76; Unconserved C terminal; Chromosome damage; Protein aggresome formation