• 作者： 黃健雄; 謝顯堂
• 作者服務機構： 美國戴維斯加州大學環境毒理學系; 國立臺灣大學農業化學系
• 中文摘要： 比較利用 Aspergillus flavus NRRL 3251 以固體醱酵及液內培養法生產黃麴毒素及之研究結果顯示：固體醱酵方式比液內培養方式適於黃麴毒素之生產，在 29°C 下以連續振盪固體培養方式培養八天時，每公斤米可生成 30 mg 之黃麴毒素及2600 mg之黃麴毒素。使用液內培養時可縮短醱酵時間，但在第五天時之最高生產量僅為每公斤米生成18 mg之黃麴毒素及1680 mg之黃麴毒素。固體培養時連續振盪有利於黃麴毒素之產生，但對黃麴毒素之生產則非必需，因此以生產黃麴毒素為目的時，可用靜置固體培養方式，以免除振盪器使用空間之限制。
• 英文摘要： Production of aflatoxins (AFM) and (AFB) by Aspergillus flavus NRRL 3251 in solid-state and shaking liquid cultures using rice as the carbon source was compared. In general, solid-statecultures produced more aflatoxins than shaking liquid cultures on an equal rice weight basis. Solid-statecultures with continuous shaking yielded higher levels of toxins than those with intermittent shaking.However, intermittent shaking is a feasible replacement for the continuous shaking method for AFMproduction. A typical solid rice culture supplemented with yeast extract produced 30 and 2600 mg per kgrice of AFM and AFB, respectively, in 8 days at 29°C. The optimal culture conditions for toxin productionin a shaking liquid culture were also studied. Parameters under consideration included the amount ofcarbon (rice) and nitrogen source, initial medium pH, and aeration rate. At optimum conditions, a represen-tative shaking liquid culture produced 18 and 1680 mg per kg rice of AFM and AFB, respectively, in 5days at 29°C. This shaking liquid culture appears feasible for scaling up and routine production of AFMand AFB for toxicological investigations.
• 中文關鍵字： afiatoxin M1; afiatoxin B1; Aspergillus flavus NRRL 3251; solid-state culture; shaking liquid culture
• 英文關鍵字： --