• 作者： 盧瀗鍹；羅銅壁
• 作者服務機構： 國立臺灣大學生化科學研究所; 中央研究院生物化學研究所
• 中文摘要： 從金色雨傘節蛇毒中三種心?毒經分離而純化並其藥理作用亦經研究。主成份心?毒（毒素VI)由118個氨基酸組成。 全部氨基酸排列次序由下述方法完成分析。原毒素先還原及羧甲基化得RCM-毒素。RCM-毒素用胰蛋白?水解，水解胜 ?片斷經離子交換，分子過濾紙層析法紙電泳法等分離純化。 RCM-毒素及全部胜?片斷利用自動氨基酸排列次序分析器 或手工Edman分析法分析氨基酸排列次序。全部胰蛋白?水解胜?片斷之重排經丁二烯酸化RCM-毒素之胰蛋白?水解 物及RCM-毒素之胰凝乳蛋白?水解物之分析而完成。原毒素由一條含有118個氨基酸而有六對分子內雙硫鍵之胜?鏈構 成。此毒素?新型心?毒素。
• 英文摘要： Three cardiotoxins have been isolated and purified from the venom of Bungarus fasciatus and their pharmacological characterization has been reported. The major cardiotoxin (Fraction vi) consists of 118 amino acids. The complete amino acid sequence was determined as follows. The native toxin was reduced and carboxymethylated (RCM-Toxin). RCM-Toxin was digested with trypsin, and tryptic peptides were isolated and purified by ion-exchange chromatography, gel filtration or by chromatography and electrophoresis on paper. RCM-Toxin and all tryptic peptides were sequenced either by automatic sequencer or by manual Edman degradation procedures. The alignment of tryptic peptides was established by the analysis of tryptic peptides of maleylated RCM-toxin and chymotryptic peptides of RCM-toxin. The native toxin consists of a single peptide chain of 118 amino acid residues intramolecularly linked by 6 disulfide bonds. This is a new . type of cardiotoxin consisting of 118 amino acids instead of 60.
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