- 作者: Jacov Tal
- 作者服務機構: Department of Virology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
- 中文摘要: --
- 英文摘要: Adeno-associated virus (AAV) vectors were shown capa-ble of high efficiency transduction of both dividing andnondividing cells and tissues. AAV-mediated transduc-tion leads to stable, long-term transgene expression inthe absence of apparent immune response.These prop-erties and the broad host range of AAV vectors indicatethat they constitute a powerful tool for gene therapy pur-poses.An additional potential benefit of AAV vectors istheir ability to integrate site-specifically in the presenceof Rep proteins which can be expressed transiently, thuslimiting their suspected adverse effects.The major re-strictions of AAV as vectors are their limited geneticcapacity and strict packaging size constraint of less than5 kb. Another difficulty is the labor-intensive and expen-sive procedure for the production and packaging ofrecombinant AAV vectors. The major benefits and draw-backs of AAV vectors and advances made in the past 3years are discussed.
- 中文關鍵字: --
- 英文關鍵字: --