- 作者: 蕭世麟;洪明奇;翁?惠;王光燦
- 作者服務機構: 國立臺灣大學生化科學研究所; 中央研究院生化研究所
- 中文摘要: 臺灣產飯匙倩蛇心?毒羧基端54-60序列之雙硫環胜?,Cys-Asn-Thr-Asp-Arg-Cys-Asn已利用固相法合成。其中脬胺酸之硫醇基分別以苯甲基及乙醯胺甲基保護,並比較二者之優劣點。 合成?物經除去保護基及氧化,純化精製後發現不具對抗心?毒之免疫活性,但由其CD光譜可以鑑定具有類似β-turn之立體構造。此與理論預測之結果相符
- 英文摘要: The C-terminal disulfide loop, Cys-Asn-Thr-Asp-Arg-Cys-Asn, consisting of residues 54 to 60 of Taiwan cobra cardiotoxin was synthesized with the solid phase method. The thiol group of cysteine was protected with benzyl or acetamidomethyl group during the synthesis, and the advantages and disadvantages of these two methods were compared. After removal of protected groups followed by oxidation and extensive purification, the synthetic peptide loop showed no antigenic activity when tested with its inhibition of precipitation against cardiotoxin-anticardiotoxin serum. However, a β-turn like structure was found on its CD spectrum. This is in accord with the result obtained by theoretical prediction.
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