• 作者： 陳忠信; 陳衍昌
• 作者服務機構： 國立台灣海洋大學水產養殖系
• 中文摘要： 以?解法製備裂片石蓴（Ulva fasciata Delile）原生質體之最適條件為以1.2M甘露醇配成4％纖維?及2％軟化?之?解
  液（滲透壓為1371.2 mOs／kg）在24℃及60rpm振盪速率下進行?解12～14小時。
  純化原生質體之方法則將原生質體一?之混合液置於35％（w／v）Ficoll-400上在200g下離心20分鐘可獲一清晰之原生質
  體中間界面層。
  以海水配成0.84M木蜜醇溶液（滲透壓1791.5 mOs／kg）對原生質體之緊迫最小。以FBA染色法追綜發現約81％之原
  生質體在24℃及166（方程式無法摘錄）照光下培養七天有81％再生細胞壁。而在逐漸更換滲透壓條件下連續培養二週則有21％原生
  質體再生為小植株或細胞團。
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• 英文摘要： A suitable condition for protoplast isolation from Ulva fasciata consisted of an enzyme combinationof 4% cellulase and 2% macerozyme in a 1.2 M sorbitol solution (osmotic pressure 1371.2 mOs/kg), overa 12-14 h exposure period at 24℃ while rotating on a shaker at 60 rpm. A procedure for purifying theseprotoplasts has been developed in which the protoplast-enzyme mixture was centrifuged with 35% (w/v)Ficoll-400 at 200 Xg for 20 minutes to obtain a clear interface of protoplast. Mannitol (0.84 M) in seawater(osmotic pressure 1791.5 mOs/kg) exhibited the least osmotic stress during the incubation of protoplasts.Using FBA Staining (Calcofluor white), we found that about 81% of the surviving protoplasts had newcell walls after seven days of incubation at 24℃ and 166 (方程式無法摘錄). Furthermore, only 21% of proto-plasts retained their ability to divide and develop into a full plant after two weeks of incubation in graduallyreduced osmotic environments.
• 中文關鍵字： protoplast; Ulva fasciata.
• 英文關鍵字： --