- 作者: 孫偉,尚智美,李清軍,焦奎
- 作者服務機構: College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China
- 中文摘要: A new quantitative method for the determination of DNA in aqueous solution based on the interaction of methyl violet (MV) with fsDNA by linear sweep voltammetric technique is proposed in this paper. The interaction of MV with fsDNA caused the decrease of the reductive peak current of MV at -0.650 V (vs. SCE) on a mercury electrode in pH 1.5 Britton-Robinson (B-R) buffer solution. Factors including the acidity of the buffer, the reaction time, the optimal concentration of MV, the reaction temperature, the influences of the foreign substances, etc. were all carefully investigated. Under the optimal conditions, the decrease of the reductive peak current of MV was proportional to the concentration of fsDNA in the linear range of 0.90~20.0 mg/L and the detection limit for fsDNA was 0.25 mg/L with the RSD of 4.00%. Synthetic samples were determined with satisfactorily results. The stoichiometry of MV with DNA was calculated by voltammetric data and the results showed that the binding ratio of DNA:MV was 1:2 with the binding constant b as 7.36 ’ 108.
- 英文摘要: --
- 中文關鍵字: Methyl violet; Nucleic acid; Linear sweep voltammetry; Interaction.
- 英文關鍵字: --