- 作者: Paul Gutwein; Anja Schramme; Mohamed SADEK Abdel-Bakky; Kai Doberstein; Ingeborg A Hauser; Andreas Ludwig; Peter Altevogt; Stefan Gauer; Anja Hillmann; Thomas Weide; Chrsitine Jespersen; Wolfgang Eberhardt; Josef Pfeilschifter
- 作者服務機構: pharmazentrum frankfurt/ZAFES, University Hospital Goethe University Frankfurt, Frankfurt am Main, Germany
- 中文摘要: --
- 英文摘要:
Background:
The importance of the Notch signaling in the development of glomerular diseases has been
recently described. Therefore we analyzed in podocytes the expression and activity of ADAM10,
one important component of the Notch signaling complex .
Methods: By Western blot, immunofluorescence and immunohistochemistry analysis we
characterized the expression of ADAM10 in human podocytes, human urine and human renal
tissue.
Results:
We present evidence, that differentiated human podocytes possessed increased amounts of
mature ADAM10 and released elevated levels of L1 adhesion molecule, one well known
substrate of ADAM10. By using specific siRNA and metalloproteinase inhibitors we demonstrate
that ADAM10 is involved in the cleavage of L1 in human podocytes. Injury of podocytes
enhanced the ADAM10 mediated cleavage of L1. In addition, we detected ADAM10 in urinary
podocytes from patients with kidney diseases and in tissue sections of normal human kidney.
Finally, we found elevated levels of ADAM10 in urinary vesicles of patients with glomerular
kidney diseases.
Conclusions:
The activity of ADAM10 in human podocytes may play an important role in the development of
glomerular kidney diseases. - 中文關鍵字: --
- 英文關鍵字: --