- 作者: Guang-wei Li; Qiu-shi Wang; Jing-hui Hao; Wen-jing Xing; Jin Guo; Hong-zhu Li; Shu-zhi Bai; Hong-xia Li; Wei-hua Zhang; Bao-feng Yang; Guang-dong Yang; Ling-yun Wu; Rui Wang; Chang-qing Xu
- 作者服務機構: Department of Pathophysiology, Qiqihar Medical University, Qiqihar, Mainland China
- 中文摘要: --
- 英文摘要:
Background: The extracellular calcium-sensing receptor (CaSR) belongs to family C of the G protein
coupled receptors. Whether the CaSR is expressed in the pulmonary artery (PA) is unknown.
Methods: The expression and distribution of CaSR were detected by RT-PCR, Western blotting and
immunofluorescence. PA tension was detected by the pulmonary arterial ring technique, and the
intracellular calcium concentration ([Ca2+]i) was detected by a laser-scanning confocal microscope.
Results: The expressions of CaSR mRNA and protein were found in both rat pulmonary artery smooth
muscle cells (PASMCs) and PAs. Increased levels of [Ca2+]o (extracellular calcium concentration) or
Gd3+ (an agonist of CaSR) induced an increase of [Ca2+]i and PAs constriction in a
concentration-dependent manner. In addition, the above-mentioned effects of Ca2+ and Gd3+ were
inhibited by U73122 (specific inhibitor of PLC), 2-APB (specific antagonist of IP3 receptor), and
thapsigargin (blocker of sarcoplasmic reticulum calcium ATPase).
Conclusions: CaSR is expressed in rat PASMCs, and is involved in regulation of PA tension by
increasing [Ca2+]i through G-PLC-IP3 pathway. - 中文關鍵字: --
- 英文關鍵字: --