- 作者: Valentina gelfanova Yein-Gei Lai Vasily Gelfanov Shiey-Cheng Tzou Yi-Fang tu Nan-Shih Liao
- 作者服務機構: Institute of Molecular Biology, Academia Sinica, Department of Zoology, National Tawiwn University and Department of Medicine, Taipei medical college, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: The immune responses of the intestine mucosa featurethe noninflammatory type, such as IgA production andoral tolerance. Th2 type cytokines have been implicatedin the induction of these noninflammatory responses. Inthe present study, cytokine responses of CD8+ and CD4+TCRαβ+ intestinal intraepithelial lymphocyte(αβ iIEL)subsets to TCR stimulation under the influence of IL-12, IL-4, or CD28 costimulation were examined. IL-12 en-hanced production of IL-10 and IFN-γ by the CD8αβ+αβiIEL significantly but only marginally affected the CD8αα+subset, whereas IL-4 induced IL-4, IL-5, and IL-10 produc-tion and augmented TGF-β production by both subsets.CD28 costimulation induced production of Th2 cytokinesby CD4+ iIEL in the absence of exogenous IL-4. Unlikelymph node CD4+ cells, the CD28 costimulation-inducedTh2 differentiation of CD4+ iIEL was not inhibited by IFN-γ. These results demonstrate active cytokine productionby CD4+, CD8αβ+, as well as CD8αα+αβ iIEL. The Th2-skewed cytokine profile of CD8αα+αβ iIEL and the IFN-γ-resistance of Th2 differentiation of the CD4+ αβ iIELsuggest that both iIEL subsets contribute to the inductionof noninflammatory mucosal immune responses.
- 中文關鍵字: --
- 英文關鍵字: Intestinal intraepithelial lymphocytes. Cytokine. Th2 responses