- 作者: 王松茂; 陳敏男; 蔡錦民; 鍾崇燊; 李宗基
- 作者服務機構: Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan 300, R.O.C.
- 中文摘要: Copper-free hemocyanin (apohemocyanin, apoHc) from Taiwan snails was prepared by cyanide treatment and the reconstitution of hemocyanin was carried out by treating apoHc with Cu(CH/sub 3/CN)/sub 4/ClO/sub 4/. At pH below 5, the apoHc solution is cloudy. When reconstituted at pH near 6, the ratio of absorbances, A/sub 345//A/sub 280/, has the normal value 0.21, and rapidly decreases with increase in pH. This indicates that recostitution is best carried out near pH 6. Addition of fluoride at pH 5.3 and cyanide at pH 5.7 causes a relatively rapid drop in absorbance at 345nm, a primary reaction followed by a slower secondary process. Comparison of the reactions of native and reconstituted hemocyanins with oxygen, fluoride, and cyanide shows that the reconstitution has been successful. Cadmium and manganese derivatives of hemocyanin have been prepared. The order of affinity of metals to the active site hemocyanin is : Cu>Mn>Cd.
- 英文摘要: --
- 中文關鍵字: Hemocyanin; copper-free hemocyanin; metal complexes.
- 英文關鍵字: Hemocyanin; copper-free hemocyanin; metal complexes.