- 作者: 眭台生 ; 蔡建勳
- 作者服務機構: 中山大學化學系
- 中文摘要: Liquid chromatography with pulsed amperometric detection (PAD) at an Au electrode was successfully applied for speciation of mercury: inorganic mercury, methylmercury and ethylmercury. The optimized conditions for triple-step potential waveforms utilized in PAD were: E/sub 1/ 1800mV, t/sub 1/ 50ms; E/sub 2/ 300mV, t/sub 2/ 3850ms; E/sub 3/ 750mV, t/sub 3/ 100ms; mercury oxidation was monitored at E/sub 3/. With the eluent of HClO/sub 4/ (0.10M)+KCl (1.0mM)+CH/sub 3/CN (1.0%) at a flow rate 0 .80mL/min, three mercury species were fully separated in 6 min on a glass column (3mm*10cm) packed with Chemcosorb C/sub 18/ (5.mu.m). However, the first peak (inorganic mercury) was commonly overlapped by the solvent front. The relative standard deviations (n=6) for methylmercury (15ppb) and ethylmercury(30ppb) were 4.2% and 2.8%, respectively. The linear range tested was 2 to 500ppb (r=0.9998) for methylmercury, and was 4 to 1000ppb (r=1.000) for ethylmercury. The detection limits (S/N=3) were 1.2ppb and 1.8ppb for methylmercury and ethylmercury, respectively. The results of determination of the mercury-containing species thimerosal in three commercial contact-lens solutions agreed satisfactorily with the expected values.
- 英文摘要: --
- 中文關鍵字: Liquid Chromatography; Pulsed Amperometric Detection; Gold Electrode; Mercury
- 英文關鍵字: 液相層析法;脈衝式電流滴定檢測;金電極;汞