- 作者: Natalia Beloborodova, Iskander Bairamov, Andrei Olenin, Victoria Shubina, Vera Teplova and Nadezhda Fedotcheva
- 作者服務機構: Negovsky Research Institute of General Reanimatology Russian Academy of Medical Sciences, Laboratory of Metabolism of Critical State, Moscow, Russia
- 中文摘要: --
- 英文摘要:
Background: Several low-molecular-weight phenolic acids are present in the blood of septic patients at
high levels. The microbial origin of the most of phenolic acids in the human body was shown
previously, but pathophysiological role of the phenolic acids is not clear. Sepsis is associated
with the excessive production of reactive oxygen species (ROS) in both the circulation and
the affected organs. In this work the influence of phenolic acids on ROS production in
mitochondria and neutrophils was investigated.
Methods: ROS production in mitochondria and neutrophils was determined by MCLA- and luminoldependent
chemiluminescence. The rate of oxygen consumption by mitochondria was determined p olarographically. The difference of electric potentials on the inner mitochondrial
membrane was registered using a TPP+-selective electrode. The formation of phenolic
metabolites in monocultures by the members of the main groups of the anaerobic human
microflora and aerobic pathogenic bacteria was investigated by the method of gas
chromatography–mass spectrometry.
Results: All phenolic acids had impact on mitochondria and neutrophils, the main producers of ROS
in tissues and circulation. Phenolic acids (benzoic and cinnamic acids) producing the prooxidant
effect on mitochondria inhibited ROS formation in neutrophils. Their effect on
mitochondria was abolished by dithiothreitol (DTT). Phenyllactate and phydroxyphenyllactate
decreased ROS production in both mitochondria and neutrophils.
Bifidobacteria and lactobacilli produced in vitro considerable amounts of phenyllactic and phydroxyphenyllactic
acids, Clostridia s. produced great quantities of phenylpropionic and phydroxyphenylpropionic
acids, p-hydroxyphenylacetic acid was produced by Pseudomonas
aeruginosa and Acinetobacter baumanii; and benzoic acid, by Serratia marcescens.
Conclusions: The most potent activators of ROS production in mitochondria are phenolic acids whose
effect is mediated via the interaction with thiol groups. Among these are benzoic and
cinnamic acids. Some phenolic acids, in particular phenyllactate and p-hydroxyphenyllactate,
which decrease ROS production in mitochondria and neutrophils, can play a role of natural
antioxidants. The results indicate that low-molecular weight phenolic acids of microbial
origin participate in the regulation of the ROS production in both the circulation and tissues,
thereby affecting the level of oxidative stress in sepsis. - 中文關鍵字: --
- 英文關鍵字: Sepsis, Phenolic acids, Mitochondria, Neutrophils, Reactive oxygen species