- 作者: Chia-Wei Chang, Hui-Chun Li, Che-Fang Hsu, Chiao-Yen Chang, Shih-Yen Lo
- 作者服務機構: Graduate Institute of Molecular and Cellular Biology, Tzu Chi University, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
To search for cellular genes up-regulated by vaccinia virus (VV) infection,
differential display-reverse transcription-polymerase chain reaction (ddRT-PCR)
assays were used to examine the expression of mRNAs from mock-infected and
VV-infected HeLa cells. Two mitochondrial genes for proteins that are part of the
electron transport chain that generates ATP, ND4 and CO II, were up-regulated after
VV infection. Up-regulation of ND4 level by VV infection was confirmed by Western
blotting analysis. Up-regulation of ND4 was reduced by the MAPK inhibitor,
apigenin, which has been demonstrated elsewhere to inhibit VV replication. The
induction of ND4 expression occurred after viral DNA replication since ara C, an
inhibitor of poxviral DNA replication, could block this induction. ATP production
was increased in the host cells after VV infection. Moreover, 4.5 μM oligomycin, an
inhibitor of ATP production, reduced the ATP level 13 hr after virus infection to that
of mock-infected cells and inhibited viral protein expression and virus production,
suggesting that increased ATP production is required for efficient VV production. Our
results further suggest that induction of ND4 expression is through a Bcl-2
independent pathway. - 中文關鍵字: --
- 英文關鍵字: --