- 作者: 陳淑姿; 蔡明世; 沈清良
- 作者服務機構: 國立成功大學醫學院解剖科; 省立臺南醫院神經外科
- 中文摘要:
FMRFamide,原名軟體動物神經胜?,分佈於軟體、節肢及各種脊椎動物的“腦-消化道”,具有調節內分泌、
自主神經及疼痛的功能,在中樞神經系統中可能作為神經傳遞物質。它在嚙齒類及靈長類的大腦分佈很廣,不過在脊椎
動物的胚胎發生情形仍然不清楚。本實驗即以大白鼠為材料,採免疫組織化學法來研究胚胎時期及出生後,前腦及間腦
部份FMRFamide的分佈及變化。
實驗結果發現,含FMRFamide的神經纖維首先出現在第18天(E18)的胚胎腦中,分佈於第三腦室周圍;神經
纖維的數量隨胚胎發育而增加,主要在下視丘,特別是弓狀核、旁室核、膈區及杏仁核等部位。另一方面,我們發現含
FMRFamide的神經元出現於胚胎出生後第一天(P ),這些神經細胞多分佈於下視丘的腹內核、無名核及基底核、杏
仁核等區域,並且隨老鼠成長而增加;在出生兩週左右,神經元數量最多,密度超過成鼠。
在前腦及間腦切片中,FMRFamide神經纖維之免疫反應出現的時間較神經細胞早,我們認為出生前的神經纖維可
能是較低位的腦幹分泌,此神經胜?在神經系統的發生上可能扮演著相當重要的角色。
e - 英文摘要: Ontogeny of the FMRFamide (molluscan cardioexcitory neuropeptide)-containing structures in theforebrain and diencephalon of the rat was investigated by employing immunohistochemical methods.FMRFamide-like immunoreacted (FMRF-IR) fibers first appeared in the borders of the periventricularzone and the preoptic area at embryonic day 18 (E18). Towaid birth, the FMRF-IR fibers gradually in-creased both in immunoreactivity and in number in these areas. A pronounced increase in FMRF-IRwas also found in the septum,the arcuate nucleus, the median eminence, the paraventricular nucleus andthe amygdaloid complex. A few FMRF-IR fibers appeared at the prenatal stage in the caudate nucleus,the bed nucleus of the stria terminalis, the dorsomedial nucleus and the cortex.The first FMRFamide-immunoreactive neurons were seen in the caudate-putamen and the amygdaloid complex at E21. TheseFMRF-IR cells increased in immunoreactivity and a significant number of cells was noted in these nucleiin the adult rat. The highest density of FMRF-IR neurons, especially in the amygdala and tuberal hypo-thalamic area, was detected at postnatal two weeks (P15). FMRFamide-like immunoreactivity in the forebrain and diencephalon appeared in the cell fibersprior to that observed in the cell bodies. This may suggest that some of the immunoreacted fibers may haveoriginated from the lower areas of the rat brain. High densities of FMRF-IR cells present in the embryonicand early postnatal stages may indicate that FMRFamide is an important factor involved in developmentalorganization of the central nervous system. These results also indicate a differential genesis of FMRF-IRneuronal groups in different regions.
- 中文關鍵字: FMRFamide; ontogeny; immunohistochemistry
- 英文關鍵字: --