- 作者: Hidekatsu Iha Hideo Tezuka So-ichi Yaguchi Kunio Tsurugi
- 作者服務機構: Deparment of Bichemistry, Institute for Experimental Animals, Yamanashi Medical University, Yamanashi, Japan
- 中文摘要: --
- 英文摘要: Fht1p is involved in the flocculation and heat tolerancemachinery of budding yeast Saccharomyces cerevisiae.Despite knowledge of its involvement in those pheno-types, a precise mechanism has yet to be discovered. Tothis end, we monitored the relationship between subcel-lular localization of Fht1p and its flocculation or heat tol-erance function using newly developed expression vec-tors with a recombinant green fluorescent protein (GFP;S65T/S147P) of Aequorea victoria added at both the N-and C-terminus of Fht1p. The main fluorescent signal ofthe GFP tagged with either a wild-type Fht1p or mutantswhich preserve their flocculation function was detectedin the nucleus, whereas signals of functionless mutantswere dispersed to the cytoplasm.
- 中文關鍵字: --
- 英文關鍵字: Fht1p/Gts1P. Green fluorescent protein (S65T/S147P). Subcellular localization. Focculation. Saccharomyces cerevisiae