- 作者: Hyang-Min Byun, Kyu Heo, Kasey J Mitchell, Allen S Yang
- 作者服務機構: Jane Anne Nohl Division of Hematology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California, USA
- 中文摘要: --
- 英文摘要:
BACKGROUND: Retrotransposons have been extensively studied in plants and animals and have been
shown to have an impact on human genome dynamics and evolution. Their ability to move within
genomes gives retrotransposons to affect genome instability.
METHODS: we examined the polymorphic inserted AluYa5, evolutionary young Alu, in the progesterone
receptor gene to determine the effects of Alu insertion on molecular environment. We used mono-allelic
inserted cell lines which carry both Alu-present and Alu-absent alleles. To determine the epigenetic
change and gene expression, we performed restriction enzyme digestion, Pyrosequencing, and Chromatin
Immunoprecipitation.
RESULTS: We observed that the polymorphic insertion of evolutionally young Alu causes increasing
levels of DNA methylation in the surrounding genomic area and generates inactive histone tail
modifications. Consequently the Alu insertion deleteriously inactivates the neighboring gene expression.
CONCLUSION: The mono-allelic Alu insertion cell line clearly showed that polymorphic inserted
repetitive elements cause the inactivation of neighboring gene expression, bringing aberrant epigenetic
changes. - 中文關鍵字: --
- 英文關鍵字: Epigenetics, Retrotransposons, Long interspersed elements, Alu