- 作者: Ping-Hsi Liaoa*, Te-Hsiang Chena and Pei-Yu Liaob
- 作者服務機構: Department of Chemical Engineering, Yung Ta Institute of Technology and Commerce, Taiwan 909, R.O.C.
- 中文摘要: The Ad2 E3-19k protein inhibits the transport of newly synthesized class I MHC molecules to the cell surface, thereby interfering with antigen presentation. The details of the interaction between E3-19k protein and class I MHC molecules have not been well-defined. In this present study, we describe the use of gel filtration HPLC for confirming the binding interaction of two domain proteins, E3-19k and MHC class I antigen, and subsequently the characterization of protein complex by SDS-PAGE. Our results demonstrate the complex formation between Ad2 lumenal E3-19k (108 amino acids, wt 108) and HLA-A*0201 molecule in vitro. Titration experiments will be employed in the future to determine stoichiometry and verify the specific interactions.
- 英文摘要: --
- 中文關鍵字: Complex formation; Adenovirus E3-19k protein; Major histocompatibility complex (MHC); HLA-A*0201.
- 英文關鍵字: Complex formation; Adenovirus E3-19k protein; Major histocompatibility complex (MHC); HLA-A*0201.