- 作者: Yu-Wei Cheng, Yung-Chun Chuang, Sheng-Wen Huang, Ching-Chuan Liu & Jen-Ren Wang
- 作者服務機構: 1.Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan 2.Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan 3.Department of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan 4.Leadgene Biomedical, Inc., Tainan, Taiwan 5.National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan 6.National Mosquito-Borne Diseases Control Research Center, National Health Research Institutes, Tainan, Taiwan 7.The Institute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan
- 中文摘要:
- 英文摘要:
Background
Enterovirus A71 (EV-A71) is a neurotropic virus which may cause severe neural complications, especially in infants and children. The clinical manifestations include hand-foot-and-mouth disease, herpangina, brainstem encephalitis, pulmonary edema, and other severe neurological diseases. Although there are some vaccines approved, the post-marketing surveillance is still unavailable. In addition, there is no antiviral drugs against EV-A71 available.
Methods
In this study, we identified a novel antibody that could inhibit viral growth through a human single chain variable fragment (scFv) library expressed in mammalian cells and panned by infection with lethal dose of EV-A71.
Results
We identified that the host protein α-enolase (ENO1) is the target of this scFv, and anti-ENO1 antibody was found to be more in mild cases than severe EV-A71 cases. Furthermore, we examined the antiviral activity in a mouse model. We found that the treatment of the identified 07-human IgG1 antibody increased the survival rate after virus challenge, and significantly decreased the viral RNA and the level of neural pathology in brain tissue.
Conclusions
Collectively, through a promising intracellular scFv library expression and screening system, we found a potential scFv/antibody which targets host protein ENO1 and can interfere with the infection of EV-A71. The results indicate that the usage and application of this antibody may offer a potential treatment against EV-A71 infection. - 中文關鍵字:
- 英文關鍵字: EV-A71, ENO1, Autoantibody, scFv