- 作者: Dan Robinson; Hua-Chien Chen; Deshan Li; Jason T. Yustein; Feng He; Wen-chang Lin; Michael J. Hayman; Hsing-Jien Kung
- 作者服務機構: a Department of Molecular Biology and Microbiology, Case Western Reserve University, School of Medicine,Cleveland, Ohio, USA; ; b Molecular and Genomic Medicine Division, National Health Research Institutes, Taipei, ; c Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC; ; d Department of Molecular Genetics and Microbiology, Life Science Building, State University of New York at Stony Brook,NY, USA
- 中文摘要: --
- 英文摘要: Tyrosine kinases are implicated in the growth and differentiation of erythroid cells. Aberrant expression and structural alterations of certain tyrosine kinases, such as erbB and sea, are known to trigger erythroleukemia development. To facilitate our understanding of the signal transduction pathways involved in erythroid differentiation and leukemic transformation, we have applied a recently developed tyrosine kinase profile technique to identify the tyrosine kinases and some novel serine/threonine kinases expressed in normal chicken erythroid progenitor cells that respond to TGFα (TGFα-EB), and erythroblasts transformed by viruses encoding v-erbB (v-erbB-EB) and v-sea (v-sea-EB). Our results reveal that the non-receptor tyrosine kinases, Abl, Fyn, Lyn, Btk and Csk, are expressed in all three cell types. The expression level of Btk, a tyrosine kinase implicated in Bruton's syndrome, is exceptionally high in the erythroblastoid cell line 6C2, transformed by the v-erbB carrying avian erythroblastosis virus, AEV-ES4. We have also uncovered a new STE-20-relat-ed serine/threonine kinase, KFC, which is abundantly expressed in both the TGFα-stimulated erythroid progenitor cells and v-sea-transformed erythroblasts. Based on sequence homology of the kinase domain, KFC appears to be the first member of a new subfamily of STE-20-like kinases.
- 中文關鍵字: --
- 英文關鍵字: erbB ; sea; TGFα; Erythroleukemia; STE-20; KFC; RT-PCR