• 作者： 王昇; 呂樹炎; 武光東
• 作者服務機構： 國立陽明醫學院共同學科; 臺北榮民總醫院大腸直腸外科
• 中文摘要： 　　人類腸癌細胞株 WiDr（來自男性）和COLO 205（來自女性）係由榮總細胞庫自美國引進。這兩個細胞株在體外的生活史已達15-20年。為了確定在長期培養中，染色體的數目和構造會有何變異，我們對每一細胞株各觀察了200個分裂中期細胞，發現WiDr有65.6％的細胞內含有68-73個染色體，而COLO 205則74％的細胞內含有70-76個染色體。WiDr雖來自男性，但在作核型分析的30個細胞中，Y染色體已完全消失。其餘的染色體－包括X染色體和體染色體－則無此種全然消失的情形。31個被作核型分析的COLO 205細胞中，其情形亦然。在兩個細胞株中，有半數以上的體染色體，在每個細胞內的平均數皆在2.0以上。一個細胞中含有5-6個同源染色體的情形亦非罕見。構造異常染色體的數目甚多。和十多年前這兩個細胞株的染色體研究報告比較，吾人發現它們的染色體數變域前增大，同時不正常的染色體數也較前增多。
• 英文摘要： A chromosomal analysis was carried out on two colorectal carcinoma cell lines (WiDr and COLO205), which were established 15-20 years ago in the US and were collected by the Cell Bank of the VeteransGeneral Hospital in Taipei. Among the 200 cells counted, 65.5% of WiDr (male) had 68-73 chromosomes,while 74% of the COLO 205 (female) had 70-76 chromosomes per cell. The Y chromosome was absent inthe 30 WiDr metaphases analyzed· None of the other chromosomes, including X and the autosomes ofboth WiDr and COLO 205, revealed such a numerical deficiency. Over half of the autosomes had an aver-age number per cell above 2.0. The existence of 5 or 6 normal homologues for certain autosomes was notrare in either line. Numerous structural abnormal marker chromosomes were present in the cells. As com-pared with the original chromosome findings which were done over 10 years ago, we noted that the rangeof chromosome counts was wider and the number of marker chromosomes increased in these long-term cul-tivated cell lines.
• 中文關鍵字： chromosome; colorectal carcinoma; human cell lines.
• 英文關鍵字： --