- 作者: Qinghua Xiong; Xiaojun Guo; Cong S. Zong; Song-muh J. Jong; Yixing Jiang; Joseph Chan; Lu-Hai Wang
- 作者服務機構: Department of Microbiology, Mount Sinai School of Medicine, New York, N.Y., USA
- 中文摘要: --
- 英文摘要: A 5,403 bp cDNA encoding chicken protein tyrosine phosphatase gamma (PTPγ) was isolated and sequenced. The predicted open reading frame of 1,422 amino acids (aa) includes 742 aa of extracellular (EC) domain, 26 aa of transmembrane (TM) domain and 634 aa of intracellular domain. The chicken PTPy has a 86.7% aa identity to its human homolog and contains the carbonic anhydrase-like domain and fibronectin type III homologous regions in the EC domain, as well as the tandem linked catalytic sequences in the cytoplasmic domain. However, the chicken PTPy lacks 29 aa immediate downstream of the putative TM domain in comparison with its human counterpart. Northern analysis revealed the presence of two transcripts of 6.3 and 9.5 kb in various tissues. The cytoplasmic domain of the PTPy could be expressed as an enzymatically active form in SF9 insect cells. PTPy could also be expressed in normal and src-transformed NIH3T3 and Rati cells as a gag-PTP fusion protein, but no detectable effects on growth and colony formation of these cells were observed.
- 中文關鍵字: --
- 英文關鍵字: PTPγ; cDNA; Cloning; Sequencing; Expression