- 作者: 陳世晞
- 作者服務機構: 中華醫專工業安全衛生科
- 中文摘要: The separation of amino acids into enantiomers while derivatizing with benzoyl chlorides is demonstrated. It was found that enantioselectivity among these reagents tagging with the same amino acid is quite different and is very sensitive to its structural variations. A noninclusion complexation mechanism, i.e. external association is thought to involve in the separation carried out with a nonaqueous polar organic mobile phase. Results also indicate that the chiral recognition seems to be controlled either by moieties from tagging reagents or functional groups on all amino acids examined in this study on the basis of competition. The polarity of side-chain group on peptide backbone can not be ignored in achieving a successful separation. Furthermore, chiral recognition appears to closely rely upon the location of stereogenic center that is simultaneously attached by amino and carboxyl groups. Finally, mechanism involved will be explored structurally with these tagging reagents derivatized amino acids which structures are highly ordered and amine-containing compounds with structures similar to that of amino acid.
- 英文摘要: --
- 中文關鍵字: Enantioresolution; Amino Acid; Tagging Reagent; Chiral Recognition Mechanism; Cyclodextrin
- 英文關鍵字: 對映解析度;胺基酸;標示劑;旋性辨識機構;環糊精