- 作者: 高景輝
- 作者服務機構: 國立臺灣大學農藝學系
- 中文摘要: 木研究係探討高粱癒傷組織之誘導以及癒傷組織在何種狀況下形成根與幼莖(shoot)o 高梁Shallu品種之種子先以氯化汞溶液消毒1分鐘,繼以10%次氯酸鈣溶液消毒20分鐘,再經無菌水清洗4-5次。消毒後之種子置於業經消毒之培養皿,於300C與黑暗狀況下發芽。六天後,在無菌狀況下切取幼苗中莖(mesocotyl)上方之部分,供癒傷組識之誘導。癒傷組識誘導之基本培養基(basal medium)為Skoog與Mura-shige二氏之配方。癒傷組識誘導之最適kinetin與2,4-D濃度分別為0.625-1·25,ug/ml與0.625-5,ug/ml o NAA與IAA不能取代2,4-D以誘導癒傷組識“在光照下,任何濃度之kinetin(1-5ug/ml )與不含auxin之培養基可誘導根之形成。癒傷組織在含有kinetin與2,4-D之培養基中繼代培養七個月仍有形成根之能力。癒傷組織幼莖形成之最適荷爾蒙條件為l,ug/ml kinetin與2·5-5,ug/ml NAA。但僅新誘導出(郎未經繼代培養)之癒傷組識才能形成幼莖。癒傷組織形成幼莖之能力隨著繼代培養很快的消失。
- 英文摘要: Induction of sorghum callus, rhizogenesis and shoot formation of callustissue from this species were studied. The concentration ranges of 0.625 to1.25,4g/ml for kinetin and 0.625 to 5 ug/ml for 2, 4-D were found to be optimalfor callus induction on the excised etiolated sorghum shoots. Root forma-tion of sorghum callus occurred in the absence of auxin at various levelsof kinetin(1-5Rg/ml)under light condition. Callus subcultured in kinetinand 2,4-D for seven months still maintained the ability to form roots.Shoot formation took place when callus was cultured in 1 ug/ml kinetinand 2.5 to 5 ug/ml NAA under light condition. Only newly induced callusshowed shoot formation. The potential of shoot formation of sorghumcallus declined rapidly by the periodic transfer to fresh culture medium.
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