- 作者: J.R. Salkowitz; B.K. Chakrabarti; B. Yen-Lieberman; C. Starkey; T. Bendele; H.W. Kestler
- 作者服務機構: a Department of Molecular Biology, Research Institute, and ; b Department of Clinical Pathology, Cleveland Clinic Foundation,Cleveland, Ohio, USA
- 中文摘要: --
- 英文摘要: AIDS viruses require an intact functional nef gene in order to inducedisease. The nonpathogenic molecular cloned virus SIVmac239nef-deletion encodes a truncated nef gene. This attenuated reading frame is expressed both in vitro and in a virus-infected animal in vivo. Encoding the first 58 amino acids of Nef, the reading frame retained its ability to down-modulate CD4 from the surface of T cells. CD4-down-modulated stable cell lines expressing full-length and truncated nef genes were significantly less infected by SIV. SIV-mac239nef-open and SIVmacnef-deletion encoding a truncated nef clearly differed in replication kinetics in H9 cells and H9-derived cell lines. SIV-mac239nef-deletion replication was delayed in H9.
- 中文關鍵字: --
- 英文關鍵字: nef; SIVmac239 ; H9; Replication ; CD4