- 作者: Chih-Yang Huang; Li-Chiu Yang; Kuan-Yu Liu; Pao-Hsin Liao; Janet Ing-Yuh Chou; Ming-Yung Chou; Wei-Wen Lin; Jaw-Ji Yang
- 作者服務機構: Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
We found that overexpression of RhoGDIβ, a Rho GDP dissociation inhibitor,
induced hypertrophic growth and suppressed cell cycle progression in a cultured cardiomyoblast cell line. Knockdown of RhoGDIβ expression by RNA interference blocked hypertrophic growth. We further demonstrated that RhoGDIβ physically interacts with ZAK and is phosphorylated by ZAK in vitro, and this phosphorylation negatively regulates RhoGDIβ functions. Moreover, the ZAK-RhoGDIβ interaction may maintain ZAK in an inactive hypophosphorylated form. These two proteins could negatively regulate one another such that ZAK suppresses RhoGDIβ functions through phosphorylation and RhoGDIβ counteracts the effects of ZAK by physical interaction. Knockdown of ZAK expression in ZAK- and RhoGDIβ-expressing cells by ZAK-specific RNA interference restored the full functions of RhoGDIβ. - 中文關鍵字: --
- 英文關鍵字: --