- 作者: 黃銓珍; 黃火鍊; 王逸琪; 蕭培文; 羅銅壁
- 作者服務機構: 中央研究院生物化學研究所; 國立台灣大學生化學研究所; 國立台灣大學動物學系
- 中文摘要: 本研究利用基因選殖、核酸序列定序與南方氏雜合試驗等,發現在白鰱、大頭鰱與草魚等三種鯉科魚類之染色體上,都存在高度重覆之Hind III鹼基序列。其中,大頭鰱之重覆Hind III鹼基序列共有兩種,而白鰱與草魚之重覆HindIII鹼基序列則只有一種;這些序列的長度有186 和201 bp兩種,而且,在染色體上呈現連續多重排列。在單套染色體上,它們的數目約為3.65 x 10,其含量約占總染色體的百分之五。白鰱、大頭鰱與草魚之染色體重覆Hind III鹼基序列彼此十分相似,但與已知的鯉魚、吳郭魚、鱈魚和鮭魚之重覆DNA序列則不同。
- 英文摘要: The genomes of three cyprinid species, silver carp, bighead carp and grass carp, all contain highlyrepetitive Hind III sequences. There are two types of repeated sequences found in the genome of bigheadcarp but only one type of repeated sequence found in the genomes of silver carp and grass carp. Theirlengths are from 186 to 201 bp. These sequences are arranged tandemly in the genomes. Their copynumbers are about 3.65 x 10 per haploid genome and total contents are about 5% of the genomes. TheHind III repetitive sequences of silver carp, bighead carp and grass carp are very similar to one anotherbut completely different from those known repetitive DNAs of common carp, tilapia, pollock and salmon.
- 中文關鍵字: repetitive DNA; chromosomal organization; copy number; cyprinidae
- 英文關鍵字: --