- 作者: Hermann M. Schatzl; Emilia Sieger; Wolfgang Jilg; Hans Nitschko; Reinhart Zachoval
- 作者服務機構: a Max von Pettenkofer Institute of Medical Virology, and ; b Department of Internal Medicine and Liver Transplantation Group1, Klinikum Grosshadern, University of Munich, and c Institute of Medical Microbiology and Hygiene, University of Regensburg,Germany
- 中文摘要: --
- 英文摘要: Variations in the major surface proteins (HBsAg) of hepatitis B virus (HBV) have been implicated in the high rate of reinfection in HBV-infected recipients of orthotopic liver transplantations (OLT). Sera from 6 OLT patients positive for HBsAg and from 3 recipients negative for it prior to transplantation were analyzed over several years, and 39 HBsAg sequences were compared. Despite anti-HBs immunoprophylaxis resulting in the disappearance of HBsAg, HBV DNA was detectable by a sensitive nested PCR in almost all sera. In 1 patient, a significant temporary shift in HBV subtypes was observed, indicating a mixed infection or the presence of multiple HBV populations in this patient; this was also true for other patients. Amino acid substitutions compared to wild-type HBV subtypes in 7 patients and variations within patients in 5 patients were detectable over time; the 'escape mutation' at amino acid position 145 was detected in 2 patients. Our data suggest that the high rate of reinfection in OLT recipients seems not to be associated with specific sequence variations in the major HBs gene, but shows a remarkable inter- and intraindividual variability. Obviously, no correlation between heterogeneity in this gene and clinical outcome was present.
- 中文關鍵字: --
- 英文關鍵字: Hepatitis B surface protein ; Genomic variability ; Escape mutation ; Liver transplantation ; Hepatitis B virus