- 作者: Po-Lin Kuo Chun-Ching Lin
- 作者服務機構: Graduate Institute of Natural Products, Kahsiung Medical University, Kaohsiung, Taiwan, ROC
- 中文摘要: --
- 英文摘要:
(-)-epigallocatechin-3-gallate (EGCG) is a polyphenolic
compound found in green tea. It has been reported to
possess a wide range of pharmacological properties,
and is one of the most promising chemopreventive
agents for cancer. To provide a better understanding of
the preventive effect of EGCG on liver cancer, we exam-
ined EGCG for its effect on proliferation and cell cycle
progression in a human liver cancer cell line, Hep G2.
The results showed that EGCG inhibited the prolifera-
tion of Hep G2 by inducing apoptosis and blocking cell
cycle progression in the G1 phase. ELISA showed that
EGCG significantly increased the expression of p53 and
p21/WAF1 protein, and this contributed to cell cycle
arrest. An enhancement in Fas/APO-1 and its two form
ligands, membrane-bound Fas ligand (mFasL) and solu-
ble Fas ligand (sFasL), as well as Bax protein, was
responsible for the apoptotic effect induced by EGCG.
Taken together, our study suggests that the induction of
p53 and the activity of the Fas/FasL apoptotic system
play major roles in the antiproliferative activity of EGCG
in Hep G2 cells. - 中文關鍵字: --
- 英文關鍵字: EGCG.p53.p21/WAF1. Fas/APO-1. Fas ligand, apoptosis