- 作者: Rong-Yaun Shyu Shiang-Long Huang Shun-Yuan Jiang
- 作者服務機構: Department of Internal Medicine, Tri-service General Hospital, Graduate Institute of Life Sciences, Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: Retinoids mediate a wide spectrum of antitumor activi-ties through induction of growth arrest, differentiation orapoptosis. To determine whether the effects of retinoidsare mediated by specific gene activation or repression,one-day treatments of SC-M1 CL23 gastric cancer cellswith vehicle alone or all-trans retinoic acid (tRA)(10μM)were compared using differential display analysis. A432-by cDNA fragment from the tRA-treated cells wasdifferentially amplified and its sequence analysis indi-cated homology with the calcium-binding protein S100P.Levels of S100P mRNA were increased 3.5-fo∣d in SC-M1CL23 gastric cancer cells treated with 10μM tRA for 1day, and the regulation was time- and concentration-dependent. Treatment with tRA (10μ) also increasedS100P mRNA levels in tRA-sensitive HtTA cells but not ininherent RA-resistant TMC-1 cells. However, the tRA-mediated increase in S100P expression was maintainedin SC-M1/R cells that were established long-term in tRA-containing medium and had acquired partial RA resis-tance to tRA-induced growth suppression. In conclusion,tRA increases S100P expression, and the regulation re-mains intact in cells which develop acquired RA resis-tance.
- 中文關鍵字: --
- 英文關鍵字: S100P. S100. Calcium-Binding Protein. Retinoic acid. Differential display. Gastric cancer