- 作者: --
- 作者服務機構: Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, 35516, Mansoura, Egypt
- 中文摘要: Three simple and sensitive spectrophotometric methods were developed for the determination of thioctic acid in bulk and in its pharmaceutical preparations using iron(III) as an oxidizing agent. Method A is based on kinetic investigation of oxidation reaction of the drug with iron(III) and a subsequent chelation of the produced iron(II) with ferricyanide to form prussian blue colored product at room temperature for a fixed time of 15 minutes at 750 nm. Methods B and C are based on oxidation of the studied drug with iron(III). The equivalent iron(II) produced is allowed to react with either o-phenanthroline or bipyridyl to give colored species measurable at 510, 522 nm, respectively. Regression analysis of Beer-Lambert plots showed a good correlation in the concentration ranges of 0.4-4 μg/mL with a detection limit of 0.095 μg/mL for method A and 0.5-5 μg/mL with detection limits 0.137 and 0.127 for method B and C, respectively. The three methods were successfully applied for the determination of the drug in its dosage forms. The percentage recoveries were 99.88 ± 1.40, 99.98 ± 1.26 and 100.64 ± 1.07, respectively.
- 英文摘要: --
- 中文關鍵字: Kinetic determination; Spectophotometry; Thioctic acid; Ferricyanide; o-Phenanthroline; Bipyridyl; Pharmaceutical analysis.
- 英文關鍵字: --