- 作者: 關媺媺; 王躬仁
- 作者服務機構: 行政院衛生署預防醫學研究所
- 中文摘要: 我們利用RT-PCR增幅臺灣1994年麻疹病毒分離株之核膜蛋白質基因,並於大腸桿菌轉殖,篩選得到一個特異限制核酸片段,再分析此基因片段與已知資料比較,結果顯示:該病毒不具有疫苗衍生株(Moranten and Schwarz vaccineassociated strains)之序列特性,亦不同於英、美等地之分離株。本RT-PCR可作為例行檢定麻疹病毒基因;並推論該病毒分離株為具有臺灣品系之遺傳特性。
- 英文摘要: Specimens were collected during a recent outbreak. Those specimens displaying both CPE positivein B95-8 lymphocyte cell culture and positive by IFA were checked by a RT-PCR with a specific set ofmeasles virus primers derived from the C-terminal of the nucleoprotein. Such RT-PCR method was foundideal for routine diagnostic purposes. Product from this RT-PCR was treated for plasmid constructionbefore transformed into E. coli. One of those transformed clones, i. e. T94,was further studied for itsDNA sequence. Since T94 is found to bear several evident different characteristics from those ever pub-lished, we conclude that this isolate is neither a vaccine derived strain nor one of those reported previouslywith specific amino acid residues, but unique in its own right. This isolate can well be a local lineage of wildmeasles virus in Taiwan.
- 中文關鍵字: measles virus; local linage.
- 英文關鍵字: --