- 作者: Yuan CY; Wang Hsu GS; Lee YJ
- 作者服務機構: Graduate Institute of Nutrition and Food Sciences, Fu-Jen Catholic University, , Taiwan, Republic of China
- 中文摘要: --
- 英文摘要:
Background: High aluminum (Al) content in certain infant formula raises the
concern of possible Al toxicity on brain development of neonates during their
vulnerable period of growing. Results of in vivo study showed that Al content of
brain tissues reached to 74 μM when oral intake up to 1110 μM, 10 times of
that in the hi-Al infant formula.
Methods: Utilizing a cultured neuron cells in vitro model, we have assessed Al
influence on neuronal specific gene expression alteration by immunoblot and
immunohistochemistry and neural proliferation rate changes by MTT assay.
Results: Microscopic images showed that the neurite outgrowth of
hippocampal neurons increased along with the Al dosages (37, 74 μM Al
(AlCl3)). MTT results also indicated that Al increased neural cell viability. On the
other hand, the immunocytochemistry staining suggested that the protein
expressions of NMDAR 1A and NMDAR 2A/B decreased with the Al dosages
(p<0.05).
Conclusion: Treated hippocampal neurons with 37 and 74 μM of Al for 14
days increased neural cell viability, but hampered NMDAR 1A and NMDAR
2A/B expressions. It was suggested that Al exposure might alter the development of hippocampal neurons in neonatal rats.
- 中文關鍵字: --
- 英文關鍵字: aluminum, neonates, primary hippocampal neuron, N-methyl-D-aspartate receptors, immunocytochemistry