• 作者： 朱廣邦; 劉立人; 王心植
• 作者服務機構： 臺北榮民總醫院醫學研究部基因重組室; 臺北榮民總醫院小兒部; 國立陽明醫學院遺傳學研究所
• 中文摘要： 為了發展適當的動物模型用於研究B型肝炎病毒（(HBV）及人類乳突瘤病毒（(HPV）其他病毒或非病毒性之因子在致癌的機制上是否有互助之作用，我們培育了含HBV, HPV16, HPV18及含癌化細胞基因之HPV16片段的基因轉殖小鼠。我們利用（C57/BL6 x CBA）F1胚，從3433枚經顯微注射及轉殖入小鼠ICR儰孕母鼠的單細胞胚中，獲181隻小鼠，經分析其中151隻小鼠，獲17隻基因轉殖小鼠。DNA互交實驗顯示在不同小鼠有1-50套基因體之轉殖，並且所分析過之小鼠皆顯示轉殖基因體的結構是頭尾銜接之多套重複體，並無Southern氏可檢測之重大改變及重組，這些基因轉殖體已穩定遺傳三代。繁殖實驗顯示，經分析的十個基因轉殖鼠系中，六隻屬於孟德爾型之遺傳，但轉殖之基因體可穿插在一至三個不同點及不同染色體，另外三個鼠系則屬多元體，第十隻則無生殖能力。
• 英文摘要： In order to produce suitable animal model systems for the analysis of possible synergistic interactionbetween hepatitis B virus (HBV) or the human papillomavirus (HPV) with other viral or chemical co-factors,we have produced transgenic mice carrying HBV, HPV16 or HPV18. Transfer of 3343 micro-injected one-cell (C57/BL6 X CBA) F1 embryos into pseudo-pregnant ICR recipients produced 181 pups. Seventeenof 151 of the pups analysed were found to be transgenics. Southern blot and slot blot analysis showedthat the copy number of the transgenome in the transgenic mice ranged from 1-50 copies per cell. All thetransgenomes analysed appeared to be in tandem repeats without detectable sequence rearrangements.The transgenome sequences were also stable on transmission to the F3 generation. On breeding, six of theten transgenic mice analysed showed the Mendelian mode of inheritance with integration at single ormultiple loci. Of the remaining four mice, three were mosaics and one was sterile．Analysis of the geneexpression of the transgenic mice is currently in progress.
• 中文關鍵字： hepatitis B virus; human papillomavirus; transgenic mice
• 英文關鍵字： --