- 作者: Ying Chu, Jiahuang Li, Xilin Wu, Zichun Hua and Zhiwei Wu
- 作者服務機構: The Center for Public Health Research, School of Medicine, Nanjing University, Nanjing 210093, Jiangsu Province, P. R. China
- 中文摘要: --
- 英文摘要:
Background: gp340, a member of scavenger receptor cysteine rich family encoded by Deleted in Malignant Brain Tumors 1 (DMBT1), is an important component in innate immune defense. The first scavenger receptor cysteine rich domain (SRCR1) of gp340 has been shown to inhibit HIV-1 infection through binding to the N-terminal flank of the V3 loop of HIV-1 gp120.
Results: Through homology modeling and docking analysis of SRCR1 to a gp120-CD4-X5 antibody complex, we identified three loop regions containing polar or acidic residues that directly interacted with gp120. To confirm the docking prediction, a series of over-lapping peptides covering the SRCR1 sequence were synthesized and analyzed by gp120-peptide binding assay. Five peptides coincide with three loop regions showed the relative high binding index. An alanine substitution scan revealed that Asp34, Asp35, Asn96 and Glu101 in two peptides with the highest binding index are the critical residues in SRCR1 interaction with gp120.
Conclusion: We pinpointed the vital gp120-binding regions in SRCR1 and narrowed down the amino acids which play critical roles in contacting with gp120.
The complete article is available as a provisional PDF. The fully formatted PDF and HTML versions are in production. - 中文關鍵字: --
- 英文關鍵字: SRCR1, DMBT1, HIV-1 gp120, Automated docking