- 作者: Jia-Qi Yao; Qing-Huai Liu; Xi Chen; Qin Yang; Zhi-Yang Xu; Fan Hu; Lin Wang; Jian-Min Li
- 作者服務機構: Department of Ophthalmology, the First Affiliated Hospital with Nanjing Medical University, China
- 中文摘要: --
- 英文摘要:
Background :
The antiproliferative effect of the Hsp90 inhibitor 17-AAG
(17-allylamino-17-demethoxygeldanamycin) on human retinal pigment epithelial cells is
investigated.
Methods :
MTT and flow cytometry were used to study the antiproliferative effects of the 17-AAG
treatment of ARPE-19 cells. 2D gel electrophoresis (2-DE) and mass spectrometry were
applied to detect the altered expression of proteins, which was verified by real-time PCR.
Gene Ontology analysis and Ingenuity Pathway Analysis (IPA) were utilized to analyze the
signaling pathways, cellular location, function, and network connections of the identified
proteins. And SOD assay was employed to confirm the analysis.
Results :
17-AAG suppressed the proliferation of ARPE-19 cells by inducing cell cycle arrest and
apoptosis. Proteomic analysis revealed that the expression of 94 proteins was altered by a
factor of more than 1.5 following exposure to 17-AAG. Of these 94, 87 proteins were
identified. Real-time PCR results indicated that Hsp90 and Hsp70, which were not identified
by proteomic analysis, were both upregulated upon 17-AAG treatment. IPA revealed that most
of the proteins have functions that are related to oxidative stress, as verified by SOD assay,
while canonical pathway analysis revealed glycolysis/gluconeogenesis.
Conclusions :
17-AAG suppressed the proliferation of ARPE-19 cells by inducing cell cycle arrest and
apoptosis, and possibly by oxidative stress. - 中文關鍵字: --
- 英文關鍵字: --