- 作者: Mariela Duarte; Kenneth Graham; Aicha Daher; Pier-Luigi Battisti; Sylvie Bannwarth; Emmanuel Segeral; Kuan-Teh Jeang; Anne Gatignol
- 作者服務機構: U332, U529 INSERM, Institut Cochin de Genetique Moleculaire, Paris, France; Molecular Virology Section, Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Md., USA; Molecular Oncology Group, McGill AIDS Centre, Lady Davis Institute for Medical Research, Montreal, QC, Canada
- 中文摘要: --
- 英文摘要: TRBP1 and TRBP2 cDNAs have been iso∣ated based onthe ability of the protein that they encode to bind HIV-1TAR RNA. The two cDNAs have different 5' end-terminiresulting in 21 additional amino acids for TRBP2 proteincompared to TRBP1. The corresponding gene is con-served in mammalian species. By PCR amplification of ahuman library, we have isolated an additional 22 nucleo-tides in the 5' end of TRBP2 cDNA. Based on the additionof these 22 new nucleotides, the first 87 nucleotides ofTRBP2 mRNA can fold into a stable stem-loop structurethat resembles TAR RNA. We have also isol ated the DNAsequence that represents the TRBP processed pseudo-gene. The absence of full alignment between TRBP2 full-length cDNA and this sequence suggests that the stem-loop structure could have prevented a complete reversetranscription during pseudogene formation. Using differ-ent antibodies, three forms of TRBP can be identified inprimate cells at 40, 43 and 50 kD, suggesting a differ-ential expression from the cDNAs and post-translationalmodifications. Both TRBP1 and TRBP2 activate the basaland the Tat-activated level of the HIV-1 LTR in humanand murine cells. Our data indicate that TRBP proteinsact at a level prior to Tat function. TRBP could contributeto improved HIV expression in murine models.
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- 英文關鍵字: --