- 作者: Robert B. Oelrichs; Victoria A. Lawson; Karen M. Coates; Catherine Chatfield; Nicholas J. Deacon; Dale A. McPhee
- 作者服務機構: AIDS Molecular and Cellular Biology Units, Macfarlane Burnet Centre for Medical Research, Fairfield, and Department of Microbiology and Immunology, University of Melbourne, Parkville, Vic., Australia
- 中文摘要: --
- 英文摘要: Two Australian HIV-1 isolates, derived from patientblood (HIV ) and cerebrospinal fluid (HIV ),were characterized after in vitro culture in peripheralblood mononuclear cells (PBMC). Although virus replica-tion was similar, as measured by cell-free reverse trans-criptase activity, only one of the two isolates(HIV-1 ) consistently induced cell syncytia and depletedthe PBMC population of CD4+ cells by cell killing. A noveltechnique, devised for rapidly obtaining high-quality vi-ral sequence data and the full-length genomic sequenceof these two isolates, is presented. Analysis of the pre-dicted sequence of the viral Env proteins provides corre-lates of the observed phenotypes. Phylogenetic analysisderived using near full-length sequence of these and oth-er HIV-1 subtype B genomic sequences(including twoother Australian isolates) shows a star-shaped phyloge-ny with each member having a similar genetic diversity.These data expand the database of genomic sequenceavailable from well-characterized primary clinical iso-lates of HIV-1 usinq a novel rapid technique.
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- 英文關鍵字: --