- 作者: Hsin-Chieh Ma; Ta-Wei Lin; Huichun Li; Sanae M. M. Iguchi-Ariga; Hiroyoshi Ariga; Yu-Li Chuang; Jing-Hsiung Ou; Shih-Yen Lo
- 作者服務機構: Graduate Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan; Graduate Institute of Medical Biotechnology,Tzu Chi University, Hualien, Taiwan; Graduate Institute of Molecular and Cellular Biology,Tzu Chi University, Hualien, Taiwan
- 中文摘要: --
- 英文摘要: The ARFP/F protein is synthesized from the +1reading frame of the hepatitis C virus (HCV) core proteingene. The function of this protein remains unknown. Tostudy the function of the HCV ARFP/F protein, we haveconducted the yeast two-hybrid screening experiment toidentify cellular proteins that may interact with the ARFP/Fprotein. MM-1, a c-Myc interacting protein, was found tointeract with HCV ARFP/F protein in this experiment. Thephysical interaction between ARFP/F and MM-1 proteinswas further confirmed by the GST pull-down assay, theco-immunoprecipitation assay and confocal microscopy.As MM-1 can inhibit the gene transactivation activity ofc-Myc, we have conducted further analysis to examine thepossible effect of the ARFP/F protein on c-Myc. Ourresults indicate that the HCV ARFP/F protein can enhancethe gene trans-activation activity of c-Myc, apparently byantagonizing the inhibitory effect of MM-1. The ability ofthe ARFP/F protein to enhance the activity of c-Myc raisesthe possibility that ARFP/F protein might play a role inhepatocellular transformation in HCV patients.
- 中文關鍵字: --
- 英文關鍵字: Hepatitis C virus; ARFP/F protein; MM-1protein; c-Myc protein; Hepatocellular transformation