- 作者: 甘魯生
- 作者服務機構: Division of Biophysics, The Johns Hopkins University, School of Hygiene and Public Health, 615N. Wolfe Street, Baltimore, MD 21205, U.S.A.
- 中文摘要: Three decadeoxyribonucleotides (d-C-C-A-A-I-A-T-T-G-G, d-C-/sup br/C-A-A-G-A-T-T-G-G and d-C-C-A-A-/sup m/G-A-T-T-G-G) involving inosine (I), 5-bromocytidine (/sup br/C) and 6-O-methylguanosine (/sup m/G were chemically synthesized based on the phosphotriester approach in milligram quantities. The procedures of full protection reaction of these uncommon or modified bases are given in detail. The phosphotriester method was then employed to synthesize all the decamers. It seems the phosphotriester method is applicable to the synthesis of oligodeoxyribonucleotide with these three odd bases. However, slightly different procedures have to be used while deblocking the fully protected oligodeoxyribonucleotides depending upon the nature of the uncommon or modified base.
- 英文摘要: --
- 中文關鍵字: Oligodeoxyribonucleotide synthesis; 5-bromocytidine; O/sup b/-methylguanosine; inosine.
- 英文關鍵字: Oligodeoxyribonucleotide synthesis; 5-bromocytidine; O/sup b/-methylguanosine; inosine.