• 作者： 劉清水; 楊居成; 周惠慈
• 作者服務機構： 國立中興大學植物系
• 中文摘要： 由木瓜組織培養苗葉肉細胞分離原生質體時，以無機鹽類作前處理之時間、細胞壁分解酵素
  （2% Driselase）處理之時間及溫度等，均與原生質體產量有關。於溫度25°C之下，以1/10MS
  培養基中之無機鹽類作前處理兩小時後，再經酵素處理5小時，可使原生質體產量達 3.7×
  cells/g．fr．wt.，較沒有前處理者產量增加7倍。
  原生質體培養於含6%甘露醇及 0.5%蔗糖之基礎液體培養基中，一個月後之原生質體存活
  量為56.3%；添加2%葡萄糖時，則可提高為205.5%。培養於含 2,4-D lppm 及 kinetin 0.2
  PPM 之培養基中，原生質體有出芽、細胞分裂等現象，於兩週後可形成由20～30個細胞組成之
  細胞?體。在培養10天後之細胞?中，經常可發現由細胞分化形成之假導管。e
• 英文摘要： To obtain the highest protoplast yield fromthe cultured papaya leaves, the followingfactors were studied: the enzyme-incubatingperiod, the temperature and the mineral salt-pretreatment time. Adding the 2-hr pretreat-ment plus the 5-hr 2% Driselase-incubatingperiod resulted the highest yield of protoplasts:3.7 x cells/g. fr. wt. The optimum tem-perature for both the pretreatment and enzymecatlaysis was 25°C. The highest survival of the one-monthprotoplasts was 56.3% if the liquid MS basalmedium supplied with 6% mannitol and 0.5%sucrose was used. The survival of the proto-plasts could be enhance to 205.5% by 2%glucose addition. The protoplast division,budding, and macrocolony were observed afterprotoplast culture for 2 and 14 days. Thetracheids also appeared in the macrocolony.
• 中文關鍵字： --
• 英文關鍵字： --