- 作者： 許織雲; 梁序穆; 葉詩雅
- 作者服務機構： 國防醫學院生物形態學系
- 中文摘要：
Cyproterone acetate (CA)係人工合成之21-C類固醇，可抑制類固醇合成?Δ -3β-HSD的活性。本實驗選用性腺
半分化族的牛蛙蝌蚪作為實驗動物。將蝌蚪分別飼於不同含量的CA水中，飼於清水中者作為對照。經10個月處理後，犧
牲蝌蚪。組織切片指示，對照蝌蚪性腺大部分為正常之卵巢，餘者為中間性別，但睪丸尚未出現。此性比例與牛蛙性腺半
分化族者相符。但經CA處理後，則全部或部分卵巢變為睪丸，其性轉變比率與CA濃度成正相關。又取蝌蚪肝臟作生化
分析，以 H-pregnenolonc為受質，測Δ -3β--HSD活性反應，顯示該?活性隨CA劑量之增加而遞減。此結果表示，在
蝌蚪卵巢性轉變過程中，干擾類固醇合成的因子可能扮演一重要的角色。
r - 英文摘要： Cyproterone acetate (CA) is a synthetic (方程式無法摘錄) steroid. It is capable of inhibiting activity of Δ -3β-HSD, which is a key enzyme for steroid biosynthesis. Tadpoles of the semidifferentiated sex race ofRana catesbeiana were reared in water containing different doses of CA. After 10 months of treatment,control tadpoles had mostly normal ovaries, some intersexes and no testes, conforming to the characteristicsof the sex race used. All ovaries of the CA-treated tadpoles were completely or partially transformed intotestes. The rate of this experimental gonadal sex transformation was dependent proportionately on CAdosage. Tadpole livers were assayed for conversion rate of Δ -3β-HSD using H-pregnenolone as the sub-strate. The results show decreased enzyme activity after CA treatment; the degree of enzyme inhibitionwas also dose-dependent. The difference between the slopes of these 2 dose-response lines is not statistical-ly significant, indicating a concomitant relationship between gonadal transformation and enzyme inhibition.Therefore, the present findings lend weight to support our suggestion that disturbance in ovarian steroido-genesis plays a role in CA-induced gonadal sex transformation.
- 中文關鍵字： --
- 英文關鍵字： --
