- 作者: Chi-Mu Chuang; Archana Monie; Annie Wu; Chien-Fu Hung
- 作者服務機構: Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
- 中文摘要: --
- 英文摘要:
Background :
It is important to develop innovative therapies for advanced stage cancers in addition to
the conventional therapies including chemotherapy, radiation and surgery.
Antigen-specific immunotherapy has emerged as a novel alternate therapy for
advanced stage cancers, which may be employed in conjunction with conventional
therapies.
Methods :
In the current study, we tested the effect of treatment with the chemotherapeutic agent,
apigenin in combination with DNA vaccines encoding the HPV-16 E7 antigen linked to
heat shock protein 70 (HSP70) in the control of the E7-expressing tumor, TC-1.
Results :
We observed that treatment with apigenin rendered the TC-1 tumor cells more
susceptible to lysis by E7-specific cytotoxic CD8+ T cells. Furthermore, treatment of
TC-1 tumor cells with apigenin was found to enhance apoptotic tumor cell death in
vitro in a dose-dependant manner. We showed that TC-1 tumor-bearing mice treated
with apigenin combined with E7-HSP70 DNA generate highest frequency of primary
and memory E7-specific CD8+ T cells, leading to potent therapeutic anti-tumor effects
against E7-expressing tumors.
Conclusions : Thus, apigenin represents a promising chemotherapeutic agent, which may be used in
combination with immunotherapy for the treatment of advanced stage cancers. The
clinical implications of the current strategy are discussed. - 中文關鍵字: --
- 英文關鍵字: --