- 作者: 朱延和
- 作者服務機構: Department of Chemistry, The Ohio State University, U.S.A.
- 中文摘要: Affinity capillary electrophoresis is a new procedure for receptor-ligand binding studies. Since its introduction in the early 1990s, this method has proved valuable in chiral separation of racemates, the measurement of binding constants, the estimation of kinetic rate constants, the determination of stoichiometries, the investigation of electrostatic interactions, the estimation of effective charges and molecular weights of biomolecules, the characterization of enzymatic catalysis, and, most recently, combinatorial library screening in solutions. This technique demands small amounts of samples, involves no radiolabeled materials or chemically immobilized ligands, and does not require changes in spectroscopic characteristics upon binding. This paper reviews the most recent applications of affinity capillary electrophoresis in binding measurement and combinatorial library screening.
- 英文摘要: --
- 中文關鍵字: Affinity Capillary Electrophoresis; Combinatorial Library Screening; Receptor Ligand Interaction; Molecular Recognition; Binding Constant; Capillary Eletrophoresis
- 英文關鍵字: 親和性毛細管電泳;結合程式庫篩選;受體配位基交互作用;分子辨證;結合常數;毛細管電泳