- 作者: 郜洪文
- 作者服務機構: State Key Laboratory of Pollution Control and Resources Reuse Research, School of Environmental Science and Engineering, Tongji University, Shanghai - 200092, P. R. China
- 中文摘要: The microphase adsorption - spectral correction (MPASC) technique has been described and applied to the aggregation of trypan blue (TB) in proteins. The formation of the microelectrostatic field in protein causes the Langmuir monolayer aggregation of TB. The adsorption ratio of TB to bovine serum albumin (BSA), ovalbumin (OVA), hemoglobin (Hb) and human g-globulin (g-G) was determined to be 14.8, 8.4, 2.8 and 27.6, respectively, and the adsorption constant of the aggregates to be 7.17 ’ 105, 4.88 ’ 106, 4.85 ’ 106 and 2.99 ’ 106. The adsorption ratio of TB to proteins interestingly indicates almost no relation to the array sequence of amino acid residues. The interaction of TB with proteins is sensitive at Ph 3.29, and the reaction was applied to the determination of protein trace with satisfactory results.
- 英文摘要: --
- 中文關鍵字: MPASC technique; Protein; Langmuir aggregation; Trypan blue (TB); Microelectrostatic field.
- 英文關鍵字: MPASC technique; Protein; Langmuir aggregation; Trypan blue (TB); Microelectrostatic field.