- 作者: S.C. Lin; M.F. Chen; C.K. Chou
- 作者服務機構: a Department of Medical Research, Veterans General Hospital;; b Graduate Institute of Microbiology and; Immunology, National Yang-Ming Medical; College, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: Two Chinese hamster ovary (CHO) cell lines stably transfected with human insulin receptor cDNA, CHO-wt and CHO-mut, which express an equivalent number of normal and kinase-defective human insulin receptors, respectively, were used to assess the roles of insulin receptor tyrosine kinase activity in insulin regulated gene expression. The effect of insulin on gene-33-promoter-driven chloramphenicol acetyltransferase (CAT), RSVLTR-driven β-galactosidase (pRSVLTR-βgal) and SV40 late-promoter-driven hepatitis B surface antigen (pMLSV2HBsAg) were examined in CHO-wt and CHO-mut cells. Insulinstimulated gene 33 promoter is 10- to 50-fold more effective in CHO-wt cells than that in parental CHO cells. However, no enhancement of insulin sensitivity of gene 33 promoter in CHO-mut cells relative to parental CHO cells was found.Similar phenomena were also observed, in that insulin regulated pRSVLTR-βgal and pMLSV2HBsAg in these three CHO lines. Our data indicated that the pro- tein kinase activity of the insulin receptor is essential for the stimulatory activity of insulin toward the activities of different promoters.
- 中文關鍵字: --
- 英文關鍵字: Insulin receptor; Protein kinase; Gene expression