- 作者: Mario Clemente Estable, Moigan H. Naghavi, Hiroyuki Kato, Hua Xiao, Jun Qin, Anders Vahlne, Robert G. Roeder
- 作者服務機構: a Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, Newyork, N.Y., b Departments of Biochemistry and Cell Biology, Bavlor College of Medicine, Houston, Tex., USA, c Division of Clinical Virology, F68, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden, d Department of Chemistry, Biology and Chemical Engineering, Ryerson University, Toronto, Canada
- 中文摘要: --
- 英文摘要: Positive transcription elongation factor-b (P-TEFb) con-tains CDK9 and cyclin T . P-TEFb was affinity purifiedfrom a stably transfected cell line that expresses epitope-tagged CDK9, and proteins that appeared to be specifi-cally bound were sequenced. In addition to CDK9, pre-viously identified isoforms of cyclin T (including T, Tand T ), HSP90 and CDC37, this analysis identified anovel protein named MCEF. Cloning of its cognate cDNArevealed that MCEF is the newest member of the AF4family of transcription factors involved in acute lympho-blastic leukemia. MCEF RNA was expressed in all humantissues examined, and antisera directed against recom-binant MCEF specifically immunoprecipitated P-TEFb.Ectopic expression of MCEF did not activate HIV-1 repli-cation, and tethering of MCEF to a promoter did not acti-vate transcription.
- 中文關鍵字: --
- 英文關鍵字: CDK9, HIV, Leukemia, Positive transcription elongation factor-b, Transcription