• 作者： Chiang, Chen-Li
• 中文摘要： 本文嘗試利用非離子型界面活性劑進行膽固醇脂解液－液兩相濁點萃取。為了找尋最適當操作條件，我們分別探討不同聚氧乙烯界面活性劑(C/sub 10/E/sub 5/, C/sub 12/E/sub 5/, and C/sub 14/E/sub 6/)、界面活性劑組成與含量、磷酸鹽濃度、溫度、二級操作對濁點溫度與酵素分離的影響。所得最適當的操作條件為：以1%(w/v)5 Lauryl Ether (C/sub 12/E/sub 5/)在22.8℃下進行，經過二級操作，可得到約5倍濃縮濃度與77%回收產率。
• 英文摘要： The use of cloud point extraction system with nonionic polyoxyethylene surfactants (C/sub 10/E/sub 5/, C/sub 12/E/sub 5/, and C/sub 14/E/sub 6/) was carried out for direct solubilization and extraction of membrane bound cholesterol esterase (cholesterol ester hydrolase; CEH) from Pseudomonas fl. The effects of various operational parameters (surfactant weight percentage, temperature, phosphate concentration and composition of surfactant mixture) on the CEH partition behavior have been investigated. The partition coefficient and yield changed insignificantly with increasing surfactant (w/v) percentage for both C/sub 12/E/sub 5/ and C/sub 14/E/sub 6/. The cloud point also shows a linear dependence on weight percentage of the shorter headgroup surfactant. The dependence is described approximately as T/sub CP/ = 20.75-0.309W and T/sub CP/= 32.82-0.243W, for C/sub 12/E/sub 4//C/sub 12/E/sub 5/ and C/sub 14/E/sub 5//C/sub 14/E/sub 6/, respectively. After a two-stage separation, an initial 5-fold concentration with 77% yield has been achieved under the condition of 1% (w/v) of C/sub 12/E/sub 5/ at 22.8.degree.C without potassium phosphate addition.
• 中文關鍵字： 濁點萃取; 膽固醇脂解; 分離係數
• 英文關鍵字： Cloud Point Extraction; Cholesterol Esterase; Partition Coefficient