- 作者: Asad U. Khan Sunil K. Lal
- 作者服務機構: Interdisciplinary Biotechnology unit, Aligarh Muslim University, Aligarh, and Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf All Road, New Delhi, India
- 中文摘要: --
- 英文摘要: Since the discovery of ribozymes and self-splicing in-trons, it has been estimated that this biological propertyof RNA combined with other recombinant DNA tech-nologies would become a tool to combat viral diseasesand control oncogenes. These goals seem like a distinctpossibility now. However, there is still a lot to be learnedabout the mobility of RNA inside the cells and the cellularfactors that can impede ribozyme action in order to capi-talize fully on the targeted RNA inactivation property ofribozymes. The most effective approach to maximizeribozyme function in a complex intracellular environ-ment is to understand as much as possible about theintracellular fate of the RNA that is being targeted.Asnew techniques in cell biology become available, suchunderstanding will be less problematic. Fundamentalstudies of ribozyme structure and mechanism of cataly-sis are flourishing both at the academic and industriallevel and it can be expected that many new develop-ments will continue to take place in these areas in thenear future. Here, we review the design,stability andtherapeutic application of these technologies illustratingrelevant gene targets and applications in molecular med-icine.Relevant problems in implementation of the tech-nology, group I and II introns and the differences in appli-cations, ribozyme structure and the application of thistechnology to virus attack and oncogene downregula-tion are discussed.Also some of the latest RNA-basedtechnologies such as siRNA, RNA/DNA duplexes andRNA decoys have been introduced.
- 中文關鍵字: --
- 英文關鍵字: Catalytic RNA. Hammerheads. Hairpins. Introns