- 作者: Khalil Farhadi*, Sedigheh Ghadamgahi, Ramin Maleki and Feridoun Salek Asgari
- 作者服務機構: Department of Chemistry, Urmia University, Urmia, Iran
- 中文摘要: A simple, sensitive and accurate spectrophotometric method has been described for the determination of ampicillin(I), amoxicillin trihydrate(II) and cefazolin sodium(III). The procedure is based on the formation of Prussian Blue (PB) complex. The reaction between the acidic hydrolysis products of antibiotics (T = 60 °C) with mixture of Fe3+ and hexacyanoferrate(III) ions was evaluated for the spectrophotometric determination of the mentioned drugs. The maximum absorbance of the colored complex occurs at l = 700 nm and the molar absorptivity is 3.0 ’ 104 l mol-1cm-1. The effect of various parameters such as concentration of K3Fe(CN)6 and Fe3+, nature and amount of acids used, temperature and time of heating were investigated. Under optimum conditions the linear range of calibration graph was 2.0-12.0, 5.0-13.5 and 3.0-12.0 mg mL-1 for ampicillin, amoxicillin and cefazolin, respectively. The relative standard deviation for the determination of 10 mg mL-1 of antibiotics was about 0.5-1.5%. The proposed method was successfully applied to the determination of selected antibiotics from pharmaceutical preparations. The validity of the method was tested by the official methods and by the recovery studies of standard addition to pharmaceuticals.
- 英文摘要: --
- 中文關鍵字: Ampicillin; Amoxicillin; Cefazolin; Prussian blue; Spectrophotometry
- 英文關鍵字: Ampicillin; Amoxicillin; Cefazolin; Prussian blue; Spectrophotometry